AAVS1 Insertion Vectors
Alstem offers two major types: reporter vectors (GFP, etc.) and customizable vectors for gene-of-interest expression. All cassettes include selectable markers and enable precise integration at the AAVS1 safe-harbor locus for stable, controlled expression using a shared gRNA/Cas9 vector.
Selection markers
- Neomycin
- Puromycin
- Hygromycin
Promoters
- Constitutive Expression
- Inducible Expression
Versatile Applications
- Reporter Expression
- Custom Gene Expression
Mechanism
The AAVS1 CRISPR/Cas9 targeting system enables precise genome modifications through the homology-directed repair (HDR) pathway. The system combines a donor vector containing homology arms and selectable transgene cassettes—reporters like GFP/RFP or customizable genes-of-interest with constitutive or inducible promoters—with a separate gRNA/Cas9 expression vector. The gRNA guides Cas9 to the AAVS1 safe-harbor locus (PPP1R12C), where Cas9 introduces a double-strand break. The donor vector serves as a template for HDR, allowing stable, selectable integration of the transgene without disrupting endogenous gene expression. This controlled system supports overexpression, rescue studies, and functional analyses, providing a platform for insights into gene function disease mechanisms, and potential therapeutics.
Selection of Edited Cells
Achieved via targeted insertion at the AAVS1 safe-harbor locus using a donor cassette containing an SA-T2A-antibiotic resistance gene, enabling stable, endogenous-level expression of the selectable marker to enrich cells with the targeted insertion.
Download System GuideApplication
Facilitates targeted insertion of genes or sequences at the AAVS1 safe-harbor locus, ensuring stable and predictable transgene expression.