Firefly luciferase is commonly used as a genetic reporter to assess the transcriptional activity of a construct with luciferase gene under the promoter of interest. We first tested the function of two different promoters, SFFV and CMV, which drive the expression of luciferase, on five different cell lines. Although luciferase activity varies in different cell lines, the SFFV promoter showed much stronger luciferase expressing than that of CMV promoter in each cell line (Figure. 1). Here, Luciferase and tdTomato dual-reporter HeLa cell line is derived from HeLa cells by transduction of ALSTEM's pLenti-SFFV-Luciferase-PGK-tdTomator-T2A-PURO Lentiviral Reporter (cat# LV452) lentivirus. The cell line stably expresses firefly luciferase at high level since it is driven by SFFV promoter. It also expresses a red fluorescent protein marker (tdTomato) and puromycin resistance gene, which are driven by PGK promoter (Figure 2). Luciferase/tdTomato dual-reporter HeLa cells can be a very useful cell line for non-invasive visualization in both in vitro and in vivo experiments.

Vector Information

Key Features:

• Excellent signal/background ratio
• Stable luciferase and tdTomato expression
• Ideal for in vivo bioluminescence imaging and cell-based assays for cancer research.

Recombinant stable cell lines are one of the widely used tools in drug discovery, toxicity testing, and basic research. Long-term stable expression of a gene of interest (GOI) is usually achieved by transfection or viral transduction of a vector containing the expression cassette of the GOI together with a selection marker (antibiotics or fluorescent proteins). In contrast to transient expression, stable cell line offers reproducible results for assay studies. Here, we packaged lentivirus and transduced a mouse cell line. The expression of transgenes was confirmed by FACS analysis Figure 4.