Lentiviral Expression Vectors
Lentiviral vectors, derived from HIV-1, are versatile tools for stable gene delivery. They efficiently transduce both dividing and non-dividing cells, making them invaluable for research and therapeutic applications. Their ability to integrate into the host genome enables long-term, stable transgene expression with low toxicity.
Alstem's versatile third-generation lentiviral backbones support stable, long-term transgene expression in dividing and non-dividing cells, with flexible promoter, reporter, and selection configurations.
Mechanism
Each vector contains a multiple cloning site (MCS) for inserting sequences before packaging.
For virus production, an expression vector is co-transfected with packaging plasmids into HEK293 cells, the gold-standard producer line. These cells generate high-titer lentiviral particles, which can then be harvested and used to transduce virtually any mammalian cell type, both in vitro and in vivo.
Safety by design
Our third-generation lentiviral system is engineered for maximum biosafety:
- Split-component design: Essential viral genes are separated across multiple plasmids.
- Replication deficient: Packaged lentivirus is replication incompetent