Inducible iPS Cell Lines
Neurons are distinct from other cell types due to their large size, functionally polarized structure, post-mitotic state, and electrochemical activity. Likewise, the human nervous system stands out for its remarkable complexity and heightened vulnerability to disease.
Current approaches for iPSC-derived neuron differentiation often face hurdles such as low efficiency, variability, and limited reproducibility. To overcome these challenges, we have developed inducible iPSC lines in which key neuronal transcription factors are integrated into the AAVS1 safe-harbor locus using CRISPR-Cas9 technology. Unlike lentiviral methods, this strategy ensures stable and precise genomic insertion, minimizing safety and variability concerns. With a Tet-on inducible system, these cell lines enable simple, rapid, and highly efficient differentiation into multi
Highlights
The inducible neuron differentiation system integrates key transcription factors at the human AAVS1 safe-harbor locus in pluripotent stem cells, enabling fast, efficient, and highly reproducible differentiation. Combined with validated protocols, these lines can reliably generate multiple neuronal subtypes for diverse research applications.
Key Features
- Stable iPSC lines engineered for rapid and efficient neuronal differentiation
- CRISPR/Cas9-mediated insertion of inducible neuronal transcription factors at the AAVS1 safe-harbor locus
- Validated differentiation protocols enabling reliable generation of multiple neuronal subtypes
Application
- Basic research
- Disease modeling
- Drug screening platform development