Lentiviral Reporter Plasmids
Lentiviral vectors, derived from human immunodeficiency virus type 1 (HIV-1), are widely used for efficient and stable gene delivery. Packaged into viral particles, they can transduce both dividing and non-dividing mammalian cells, making them powerful tools for research and therapeutic applications. Our premade lentiviral reporter plasmids are designed for plug-and-play use, allowing rapid creation of stable cell lines with integrated reporter cassettes for labeling, functional assays, or imaging studies.
Mechanism
Lentiviral vectors function by integrating transgenes into the host genome, ensuring stable, long-term expression. They efficiently infect both proliferating and quiescent cells with low immunogenicity and minimal toxicity. These reporter plasmids are used together with packaging plasmids and HEK293 cells to generate high-titer lentiviral particles. The viruses are typically pseudotyped with VSV-G, enabling broad cell tropism and efficient transduction across a wide range of mammalian cells. By integrating reporter cassettes (e.g., GFP, RFP, luciferase, NanoLuc) into the genome, researchers can establish stable reporter cell lines for various applications such as cell labeling, in vivo imaging, tumor evaluation, or drug screening.
Safety
Our platform uses a third-generation lentiviral system, engineered for enhanced biosafety. Viral functions are separated across multiple helper plasmids to prevent formation of replication-competent viruses. This design allows researchers to generate functional lentiviral particles safely and reliably for both in vitro and preclinical studies.
Promoter & Reporter Options
We offer a variety of premade reporter constructs with different promoters, reporters, and selection markers to suit diverse experimental requirements.
| Promoter | Reporter | Selection |
|---|---|---|
| EF1 | GFP | Puromycin |
| CMV | RFP | Neomycin |
| CAG | Luciferase | Hygromycin |
| SFFV | NanoLuc® | Blasticidin |
| LgBiT | ||
| SmBiT |
Note: These ready-to-use plasmids, together with packaging components and HEK293 cells, allow generation of high-titer, VSV-G pseudotyped lentiviruses for efficient establishment of stable cell lines across a wide variety of mammalian cells.