Knockout gRNA Vectors
Plasmid-based vectors co-expressing Cas9 (CBh promoter) and pre-cloned, validated gRNAs (U6 promoter) for high-efficiency genome editing. Selectable after transfection and usable individually or in pairs for streamlined knockout experiments.
Validated Performance
- Experimentally validated for high cutting efficiency
- Optimized specificity for reliable genome editing
Flexible Applications
- Generate small deletions causing frameshift mutations
- gRNAs used individually or paired
All in one design
- Co-expresses SpCas9 protein and pre-cloned gRNA
- Includes selectable marker for transfection-based selection
Mechanism
Pre-validated gRNAs with high cutting efficiency and specificity are cloned into all-in-one plasmid vectors. The U6 promoter drives gRNA expression, while the CBh promoter controls SpCas9 and a selectable marker (e.g., puromycin). Single or paired gRNAs induce indels, potentially causing frameshifts, premature termination of the coding sequence, or production of nonfunctional proteins.
Application
Gene Knockout & Functional Studies
- Generating frameshift knockouts: introduces insertions or deletions that disrupt the reading frame, effectively inactivating the gene.
- Functional genomics: study gene function, regulatory networks, and molecular pathways.
Biomedical Research & Disease Modeling
- Investigation of genetic diseases and disorders.
- Development of cellular or animal models for understanding disease mechanisms.
- Screening for potential therapeutic targets.
Biotechnology & Synthetic Biology
- Creation or optimization of biotechnological products in agriculture, industry, or synthetic biology.
- Engineering of microbial, plant, or mammalian systems for desired traits.
Drug Discovery & Therapeutic Development
- Validation of drug targets by gene knockout.
- Testing gene function in response to small molecules or biologics.
- Exploration of novel treatment strategies through targeted gene disruption.